Development of simple sequence repeat markers for Japanese isolates of Magnaporthe grisea

作者：Suzuki F^*; Suga H; Tomimura K; Fuji S; Arai M; Koba A; Nakajima T

来源：Molecular Ecology Resources, 2009, 9(2): 588-590.

DOI：10.1111/j.1755-0998.2008.02446.x

• bioinformatics
• population genetics
• rice blast
• SSR

We developed nine simple sequence repeat (SSR) markers useful for differentiating Japanese isolates of Magnaporthe grisea through a bioinformatic approach. Repeat sequences in the genome of M. grisea were identified by a Tandem Repeat Finding program. Length polymorphisms at 28 loci were examined, nine of which were selected on the basis of detected polymorphisms. These nine SSR markers showed a Nei's gene diversity ranging from 0.23 to 0.91 among 48 field isolates of two natural populations. These SSR markers are well suited for M. grisea epidemiology and population genetics.

• 出版日期2009-3