Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

作者：Dai, Lingzi; Guo, Nian; Liu, Yaqin; Shen, Shanshan; Ge, Qiufu; Pan, Yuanjiang^*

来源：Chinese Chemical Letters, 2019, 30(1): 103-106.

DOI：10.1016/j.cclet.2017.12.023

• High-performance liquid chromatography/tandem mass spectrometry
• NL-101
• Amino acids
• Peptides
• Binding sites

The binding between NL-101, a novel nitrogen mustard anti-cancer drug, with amino acids and peptides has been investigated by high performance liquid chromatography electrospray tandem mass spectrometry (HPLC/ESI-MS/MS). This study offers supporting data of the interaction among drug and amino acids and peptides, which could potentially explain the cytotoxic and mutagenic effects of the drug. Collision-induced dissociation (CID) experiment demonstrated that under the same collision energy, the amino group combined with NL-101 adducts are sensitive and often produce more fragment ions: the carboxyl group combined with NL-101 adducts are hard to break and display fewer fragment ions. In addition, when other group (like sulfhydryl group) of amino acids binds to NL-101, CID spectra show different fragmentation pattern. These differences could display structural information about the drug adducts and be utilized as location of the authentic binding sites.

• 出版日期2019-1
• 单位浙江大学