Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

作者：Jordan Nan Yeun; Mimpen Jolet Y; van den Bogaard Willie J M; Flesch Frits M; van de Meent Michiel H M; Torano Javier Sastre^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2015, 995: 70-73.

DOI：10.1016/j.jchromb.2015.05.020

• Caffeine
• Paraxanthine
• CYP1A2
• Phenotyping
• Saliva
• UHPLC

Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions <13.5%. The limits of detection were 0.16 and 0.63 ng mL(-1) for PX and CAF, respectively. PX/CAF concentration ratios from volunteers were 0.26-1.09 with mean ratios of 0.78 +/- 0.26 and 0.38 +/- 0.10 for regular and light/non-coffee drinkers, respectively.

• 出版日期2015-7-15