Adaptation of the [H-3]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites

作者：Arnold Megan S J; Engel Jessica A; Chua Ming Jang; Fisher Gillian M; Skinner Adams Tina S; Andrews Katherine T^*

来源：Antimicrobial Agents and Chemotherapy, 2016, 60(7): 4361-4363.

DOI：10.1128/AAC.02948-15

The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [H-3]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC(50)s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery.

• 出版日期2016-7