Activity of three antiviral drugs against bovine herpesviruses 1, 2 and 5 in cell culture

作者：Dezengrini Renata; da Silva Sara C; Weiss Marcelo; Kreutz Luiz C; Weiblen Rudi; Flores Eduardo F^*

来源：Pesquisa Veterinaria Brasileira, 2010, 30(10): 855-860.

DOI：10.1590/S0100-736X2010001000008

• Antivirals
• BoHV-1
• BoHV-2
• BoHV-5
• Foscarnet
• Gancyclovir
• Acyclovir

Dezengrini R., Silva S.C., Weiss M., Kreutz L.C., Weiblen R. & Flores E.F. 2010. [Activity of three antiviral drugs against bovine herpesviruses 1, 2 and 5 in cell culture.] Atividade de tres drogas antivirais sobre os herpesvirus bovino tipos 1, 2 e 5 em cultivo celular. Pesquisa Veterinaria Brasileira 30(10): 855-860. Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Centro de Ciencias Rurais, Universidade Federal de Santa Maria, Av. Roraima 1000, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: [e-mail]
The activity of three anti-herpetic drugs (Acyclovir [ACV], Gancyclovir [GCV] and Foscarnet [PFA]) was tested against bovine herpesvirus 1 (BoHV-1), 2 (BoHV-2) and 5 (BoHV-5) in vitro using the plaque reduction assay. Different drug concentrations were tested against one hundred 50% tissue culture infectious dose (TCID(50)) of the respective viruses. Drug concentrations lower than 200 mu g/mL resulted in viability rates of more than 80% for MDBK and Hep2 cells in the MTT test (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The selectivity index (IS) of the drugs was calculated dividing the concentration of the drug that is cytotoxic for 50% of the cells (CC 50) by the concentration of the drug that was effective in reducing by 50% the number of viral plaques (EC(50)) for the three herpesviruses. Thus, ACV was shown to be moderately active against BoHV-1 (EC(50) : 112.9 mu g/mL; IS: 4.5), BoHV-2 (EC(50) : 114.2 mu g/mL; IS: 4.5) and BoHV-5 (EC(50) : 96.9 mu g/mL; IS: 5.3). GCV was effective against BoHV-2 (EC(50) : 33.5 mu g/mL; IS: 16.6), moderately effective against BoHV-5 (EC(50) : 123.2 mu g/mL; IS: 4.5) and poorly active against BoHV-1 (EC(50) : 335.8 mu g/mL; IS: 1.7). PFA exhibited the highest antiviral activity, being the only drug that, at concentration of 100 mu g/mL, completely inhibited plaque formation by all three viruses. PFA was the most effective in vitro against BoHV-1 (EC(50) : 29.5 mu g/mL; IS: 42.2), BoHV-2 (EC 50 : 45.2 mu g/mL; IS: 27.6) and BoHV-5 (EC(50) : 7.8 mu g/mL; IS: 160.6). Thus, the results indicate that PFA is a promising candidate for experimental therapeutic testing in vivo against bovine herpesviruses.

• 出版日期2010-10