New drug-eluting lenses to be applied as bandages after keratoprosthesis implantation

作者：Carreira A S; Ferreira P^*; Ribeiro M P; Correia T R; Coutinho P; Correia I J; Gil M H

来源：International Journal of Pharmaceutics, 2014, 477(1-2): 218-226.

DOI：10.1016/j.ijpharm.2014.10.037

• Drug-eluting lens
• Keratoprosthesis
• Poly(vinyl alcohol)
• Chitosan
• Glyoxal

Corneal tissue is the most commonly transplanted tissue worldwide. This work aimed to develop a new drug-eluting contact lens that may be used as a bandage after keratoprosthesis. During this work, films were produced using poly(vinyl alcohol) (PVA) and chitosan (CS) crosslinked with glyoxal (GL). Vancomycin chlorhydrate (VA) was impregnated in these systems by soaking. Attenuated total reflectance - Fourier transform infrared spectroscopy was used to confirm crosslinking. The cytotoxic and drug release profile, hydrophilicity, thermal and biodegradation as well as swelling capacity of the samples were assessed through in vitro studies. PVA and PVA/CS films were obtained by crosslinking with GL. The films were transparent, flexible with smooth surfaces, hydrophilic and able to load and release vancomycin for more than 8 h. Biodegradation in artificial lachrymal fluid (ALF) with lysozyme at 37 degrees C showed that mass loss was higher for the samples containing CS. Also, the samples prepared with CS showed the formation of pores which were visualized by SEM. All samples revealed a biocompatible character after 24 h in contact with cornea endothelial cells. As a general conclusion it was possible to determine that the 70PVA/30CS film showed to combine the necessary features to prepare vancomycin-eluting contact lenses to prevent inflammation after corneal substitution.

• 出版日期2014-12-30