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摘要
Although exposure to environmental pollutants is one of the risk factors for renal cell carcinoma (RCC), its relationship with carcinogenesis and the progression of RCC remains unknown. The present study was designed to elucidate the role of the aryl hydrocarbon receptor (AhR), a major mediator of carcinogenesis caused by environmental pollutants, in the progression of RCC. The expression of AhR was investigated in 120 patients with RCC using immunohistochemistry, and its relationship with clinicopathological parameters and prognoses was statistically analyzed. RCC cell lines were exposed to indirubin or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), AhR ligands, to activate the AhR pathway, or were transfected with small interfering RNA (siRNA) for AhR. The expression of the AhR target genes CYP1A1 and CYP1B1, matrix metalloproteinases (MMPs), and invasion through Matrigel(TM) were then examined. AhR was predominantly expressed in the nuclei of high-grade clear cell RCC (ccRCC) and tumor-infiltrating lymphocytes (TILs), and its expression levels in cancer cells and TILs correlated with the pathological tumor stage and histological grade. A multivariate Cox analysis revealed that the strong expression of AhR in cancer cells was a significant and independent predictor of disease-specific survival. AhR ligands up-regulated the expression of AhR and CYPs and promoted invasion by up-regulating MMPs. Furthermore, siRNA for AhR down-regulated CYPs, and inhibited cancer cell invasion together with the down-regulation of MMPs. These results suggest that AhR regulates the invasion of ccRCC and may be involved in tumor immunity. Therefore, inhibiting the activation of AhR may represent a potentially attractive therapeutic target for ccRCC patients. What's new? As excretory organs, the kidneys are prime sites of exposure to carcinogenic substances, particularly environmental pollutants. But while such pollutants are known risk factors for clear cell renal cell cancer (ccRCC), little is known about their relationship with ccRCC progression. In this study, expression of the aryl hydrocarbon receptor (AhR), a mediator of pollutant-associated carcinogenesis, was detected in high-grade ccRCCs and tumor-infiltrating lymphocytes. In cancer cells and lymphocytes, AhR expression levels were associated with pathological tumor stage, histological grade and poor prognosis. AhR activation was found to enhance ccRCC invasiveness, while AhR silencing via siRNA inhibited invasion in vitro.
- 出版日期2015-7-15
