A novel culture system for the ultra-high-density production of the rotifer, Brachionus rotundiformis - a preliminary report

作者：Yoshimura K; Tanaka K; Yoshimatsu T^*

来源：Aquaculture, 2003, 227(1-4): 165-172.

DOI：10.1016/S0044-8486(03)00501-5

• rotifer
• culture
• high density
• membrane filter
• ammonia

A novel culture system for continuous filtration of culture water was developed for ultra-high-density production of a marine rotifer. This ultra-high-density culture system is equipped with a membrane filtration unit (pore size: 0.4 mum) set inside a culture vessel. The culture performance of this system was tested by feeding freshwater Chlorella paste as feed in a 4-day batch culture.
To prevent the membrane surface from fouling, the filtration unit was cleaned every day by dipping it in a NaOCl solution. The membrane filter unit was effective in exchanging the culture water with a high level of ammonia with fresh seawater, while rotifers and Chlorella cells were retained in the vessel. Using this membrane filtration unit with the supply of oxygen gas, a rotifer density of more than 1.6 x 10(5) individuals ml(-1) was obtained on the 4th day of culture. The removal of accumulated ammonia from the culture water enabled an increase of the productivity and of the vitality of the rotifers.

• 出版日期2003-11-10