科研之友
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摘要
T helper (Th) 17 cells are difficult to isolate which hinders experimental studies with these cells. Here, we report a novel method to obtain sortable, engineered mouse Th17 cells. First, we developed lentiviral vector (XZ12) containing ROR gamma t gene and mouse Delta NGFR gene complemented with IL17A promoter (pXZ12-ROR gamma t). As control, we used vector pXZ12 containing mouse Delta NGFR gene complemented with IL17A promoter. Ieouse CD4(+)CD25(-) T cells were transduced with pXZ12-ROR gamma t or pXZ12 vectors and cultured in the presence of transforming growth factor (TGF)-beta or interleukin (IL)-6. Then, we isolated Th17 cells using anti-Delta NGFR magnetic beads. The cytokine production profiles of isolated Th17 cells were assessed by qPCR, while cell functional capabilities tested in an experimental model of mouse autoimmune encephalomyelitis (EAE). We observed that overexpression of ROR gamma t in the presence of TGF-beta and IL-6 is highly efficient to produce Th17 cells. After sorting, the purity of IL-17A(+) population was over 90 %. The phenotype of pXZ12-ROR gamma t transduced cells in the presence of TGF-beta and IL-6 was similar to natural Th17 cells, in contrast to cells overexpressing ROR gamma t alone which were deficient for IL-21. The engineered Th17 cells intensified EAE in C57BL6 mice indicating that these cells were phenotypically and functionally similar to natural Th17 cells. In conclusion, the engineered Th17 cells described here can be a useful tool to advance studies on Th17 cells.
