科研之友
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摘要
A nano-micelle with highly efficient peroxide activity was constructed by self-assembly of sodium dodecyl sulfate micellar, histidine and hematin in 50 mM phosphate buffer at 25 A degrees C. UV-Vis spectrometry methods were utilized for characterization of the nanostructured material or artificial peroxidase (AP). The Michaelis-Menten (K (m)) and catalytic rate (k (cat)) constants of the AP were obtained to be 5.5 mu M and 0.06 s(-1), respectively, in 50 mM phosphate buffer solution at pH 8.0. The catalytic efficiency of AP was evaluated to be 0.011 mu M-1 s(-1). The AP was also immobilized on a functional multi-wall carbon nanotubes-gold nanoparticles (AuNPs) nano-complex modified glassy carbon electrode (GCE). The transmission electron microscopy method was utilized for the characterization of the nano-materials. The electron-transfer rate constant (k (s)) and the apparent Michaelis-Menten constant K (m) (app) of the AP modified GCE were evaluated to be 1.36 s(-1) and 0.19 mu M, respectively. For a biosensor without a redox protein, the properties of the AP modified GCE were significant and will further benefit from additional studies and improvement.
- 出版日期2014-10
- 单位河南大学
