Amyloid beta oligomers induce neuronal elasticity changes in age-dependent manner: a force spectroscopy study on living hippocampal neurons

作者：Ungureanu Andreea Alexandra^*; Benilova Iryna; Krylychkina Olga; Braeken Dries; De Strooper Bart; Van Haesendonck Chris; Dotti Carlos G; Bartic Carmen

来源：Scientific Reports, 2016, 6(1): 25841.

DOI：10.1038/srep25841

Small soluble species of amyloid-beta (A beta) formed during early peptide aggregation stages are responsible for several neurotoxic mechanisms relevant to the pathology of Alzheimer's disease (AD), although their interaction with the neuronal membrane is not completely understood. This study quantifies the changes in the neuronal membrane elasticity induced by treatment with the two most common A beta isoforms found in AD brains: A beta 40 and A beta 42. Using quantitative atomic force microscopy (AFM), we measured for the first time the static elastic modulus of living primary hippocampal neurons treated with pre-aggregated A beta 40 and A beta 42 soluble species. Our AFM results demonstrate changes in the elasticity of young, mature and aged neurons treated for a short time with the two A beta species pre-aggregated for 2 hours. Neurons aging under stress conditions, showing aging hallmarks, are the most susceptible to amyloid binding and show the largest decrease in membrane stiffness upon A beta treatment. Membrane stiffness defines the way in which cells respond to mechanical forces in their environment and has been shown to be important for processes such as gene expression, ion-channel gating and neurotransmitter vesicle transport. Thus, one can expect that changes in neuronal membrane elasticity might directly induce functional changes related to neurodegeneration.

• 出版日期2016-5-13