科研之友
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摘要
This paper investigates the biological reactions of unicellular creature irradiated to a low intensity of ultrasonic field. The current study attempts to anticipate the value of the ultrasonic driving frequency which will induce the most significant biological reactions by using the theoretical model. The theoretical model of the cells in response to the ultrasonic irradiation is simulated using Rayleigh-Plesset's bubble activation theory. The simulation results indicate that the resonant frequency of the Paramecium vacuoles considered in the present study lies in the range 0.54-1.24MHz. Ultrasonic irradiation experiments are performed at various power level intensities at driving frequencies corresponding to resonant (0.5 and 1 MHz) and nonresonant (0.25 and 5MHz) frequencies. It is found that samples irradiated under different ultrasonic conditions exhibit clear differences in their cell proliferation tendencies. For example, in samples irradiated with lower power intensities and driving frequencies of 0.5 and 1 MHz, the number of cells in the treated samples is found to be approximately 30% higher than that in the original unexposed samples. However, when resonant frequencies and higher intensities are applied, the ultrasonic irradiation causes a shape change of the cell organelles and a corresponding reduction in the total number of cells in the treated sample. For the samples exposed to nonresonant frequency ultrasonic irradiation, it is found that the cell proliferation is limited and appears to vary independently of the applied irradiation intensity.
- 出版日期2008-12
- 单位中山大学
