A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1

作者：Orikasa Yoshitake; Nishida Takanori; Hase Akira; Watanabe Kazuo; Morita Naoki; Okuyama Hidetoshi^*

来源：FEBS Letters, 2006, 580(18): 4423-4429.

DOI：10.1016/j.febslet.2006.07.008

• docosahexaenoic acid
• eicosapentaenoic acid
• n-3 polyunsaturated fatty acid
• phosphopantetheinyl transferase
• pfaE gene
• Moritella marina strain MP-1

A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA-D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n - 3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-I.

• 出版日期2006-8-7