Apelin receptor homodimer-oligomers revealed by singlemolecule imaging and novel G protein-dependent signaling

作者：Cai, Xin; Bai, Bo^*; Zhang, Rumin; Wang, Chunmei; Chen, Jing^*

来源：Scientific Reports, 2017, 7(1): 40335.

DOI：10.1038/srep40335

• REFLECTION FLUORESCENCE MICROSCOPY
• CARDIAC-HYPERTROPHY
• LIVING CELLS
• HETERODIMERIZATION
• IDENTIFICATION
• TRANSDUCTION
• DIMERS
• HETEROMERIZATION
• PHOSPHORYLATION
• HETEROTETRAMER

The apelin receptor (APJ) belongs to family A of the G protein-coupled receptors (GPCRs) and is a potential pharmacotherapeutic target for heart failure, hypertension, and other cardiovascular diseases. There is evidence APJ heterodimerizes with other GPCRs; however, the existence of APJ homodimers and oligomers remains to be investigated. Here, we measured APJ monomer-homodimer-oligomer interconversion by monitoring APJ dynamically on cells and compared their proportions, spatial arrangement, and mobility using total internal reflection fluorescence microscopy, resonance energy transfer, and proximity biotinylation. In cells with <0.3 receptor particles/mu m(2), approximately 60% of APJ molecules were present as dimers or oligomers. APJ dimers were present on the cell surface in a dynamic equilibrium with constant formation and dissociation of receptor complexes. Furthermore, we applied interference peptides and MALDI-TOF mass spectrometry to confirm APJ homo-dimer and explore the dimer-interfaces. Peptides corresponding to transmembrane domain (TMD)1, 2, 3, and 4, but not TMD5, 6, and 7, disrupted APJ dimerization. APJ mutants in TMD1 and TMD2 also decreased bioluminescence resonance energy transfer of APJ dimer. APJ dimerization resulted in novel functional characteristics, such as a distinct G-protein binding profile and cell responses after agonist stimulation. Thus, dimerization may serve as a unique mechanism for fine-tuning APJ-mediated functions.

• 出版日期2017-1-16
• 单位济宁医学院; 山东大学