Nortriptyline protects testes against germ cell apoptosis and oxidative stress induced by testicular ischaemia/reperfusion

作者：Yazdani I; Ghazi Khansari M; Saravi S S Saeedi; Nobakht M; Majdani R; Rezayat S M; Mousavi S E; Yari A; Dehpour A R^*

来源：Andrologia, 2017, 49(2): e12605.

DOI：10.1111/and.12605

• Apoptosis
• mitochondrial permeability transition pore
• nortriptyline
• oxidative stress
• testicular T/D

We designed this experiment to evaluate the effects of nortriptyline on testicular injury after torsion/detorsion (T/D). Ninety-six adult Wistar rats were divided into six groups 16 each in control group (Group 1), sham operated (Group 2), T/D+saline (Group 3), and in groups 4-6; were administered 2, 10 and 20mg kg(-1), i.p. of nortriptyline 30 and 90min after torsion respectively. Testicular torsion was created by twisting the right testis 720 degrees in clockwise direction for 1h. In six rats of each group, tissue MDA level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in compared with control group 4h after detorsion (P<0.001). In six rats of each group 24h after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubules diameters (MSTD) and TUNEL test. Moreover, 30days after T/D, sperm concentration and motility were examined in rest of rats.
Pre- and post-reperfusion nortriptyline could reduce MDA and caspase-3 levels and normalise antioxidant enzymes activities, dose dependently. Germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, were significantly improved. Inhibition of mitochondrial permeability transition pore is probably involved in protective effects of nortriptyline against testicular T/D cell damages.

• 出版日期2017-3