A cholesterol biosensor based on a bi-enzyme immobilized on conducting poly(thionine) film

作者：Rahman Md Mahbubur; Li Xiao bo; Kim Jaecheon; Lim Beong Ou; Ahammad A J Saleh; Lee Jae Joon^*

来源：Sensors and Actuators B: Chemical , 2014, 202: 536-542.

DOI：10.1016/j.snb.2014.05.114

• Poly(thionine)
• Cholesterol biosensor
• Cholesterol oxidase
• Horseradish peroxidase
• Hydroquinone

A simple and cheap cholesterol biosensor was designed by immobilizing cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) onto a poly(thionine)-modified glassy carbon electrode (GCE/PTH). Being mediated by hydroquinone (HQ), the immobilized HRP exhibited excellent electrocatalytic activity in reducing H2O2, which was produced from cholesterol by the enzymatic reaction of ChOx. The linear detection range for cholesterol was 25-125 mu M, with a detection limit (S/N = 3) and a sensitivity of 6.3 mu M and 0.18 mu A/cm(2)/mu M, respectively, under optimal conditions. The highly reproducible and sensitive GCE/PTH/ChOx/HRP sensor exhibited an interference-free signal for cholesterol detection with excellent recoveries for real sample analysis.

• 出版日期2014-10