A minimal length rigid helical peptide motif allows rational design of modular surfactants

作者：Mondal Sudipta; Varenik Maxim; Bloch Daniel Nir; Atsmon Raz Yoav; Jacoby Guy; Adler Abramovich Lihi; Shimon Linda J W; Beck Roy; Miller Yifat; Regev Oren; Gazit Ehud

来源：Nature Communications, 2017, 8(1): 14018.

DOI：10.1038/ncomms14018

Extensive work has been invested in the design of bio-inspired peptide emulsifiers. Yet, none of the formulated surfactants were based on the utilization of the robust conformation and self-assembly tendencies presented by the hydrophobins, which exhibited highest surface activity among all known proteins. Here we show that a minimalist design scheme could be employed to fabricate rigid helical peptides to mimic the rigid conformation and the helical amphipathic organization. These designer building blocks, containing natural non-coded a-aminoisobutyric acid (Aib), form superhelical assemblies as confirmed by crystallography and microscopy. The peptide sequence is amenable to structural modularity and provides the highest stable emulsions reported so far for peptide and protein emulsifiers. Moreover, we establish the ability of short peptides to perform the dual functions of emulsifiers and thickeners, a feature that typically requires synergistic effects of surfactants and polysaccharides. This work provides a different paradigm for the molecular engineering of bioemulsifiers.

• 出版日期2017-1-13