An investigation of the interactions between an E-coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

作者：Qin Xiaofei; Engwer Christoph; Desai Saaketh; Vila Sanjurjo Celina; Goycoolea Francisco M

来源：Colloids and Surfaces B: Biointerfaces , 2017, 149: 358-368.

DOI：10.1016/j.colsurfb.2016.10.031

• Nanocapsules
• Chitosan
• Quorum sensing
• Bacteria
• Interactions

We examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter similar to 114-155 nm, polydispersity similar to 0.127, and zeta-potential similar to+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of similar to 80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and zeta-potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the "stoichiometric" ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.

• 出版日期2017-1-1