Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase-amidase cascade system of Microbacterium imperiale CBS 498-74

作者：Cantarella L; Gallifuoco A; Malandra A; Martinkova L; Pasquarelli F; Spera A; Cantarella M^*

来源：Enzyme and Microbial Technology, 2010, 47(3): 64-70.

DOI：10.1016/j.enzmictec.2010.05.009

• Nitrile hydratase-amidase cascade system
• 3-Cyanopyridine bioconversion
• Nicotinamide
• Nicotinic acid
• Continuous stirred membrane reactor

The bioconversion of 3-cyanopyridine using the in situ nitrile hydratase-amidase cascade system of resting Microbacterium imperiale CBS 498-74 cells was investigated in an ultrafiltration-membrane reactor, operated in either batch or continuous mode. The effects of operating conditions such as the amount of biocatalyst, substrate concentration, substrate feeding rate, mean residence time, and enzyme-to-substrate ratio, were investigated with the aim of achieving almost 100% substrate conversion and high reactor productivity. As a result, it was found that the NHase-AMase cascade system could be adequately exploited in a continuous reactor configuration. The differing temperature dependence of nitrile hydratase and amidase kinetics enabled the operational parameters to be module d to ensure (i) nitrile hydratase operational stability (at 5 degrees C), and (ii) 100% conversion of 3-cyanopyridine into nicotinic acid, or, alternatively, (iii) enrichment of the effluent stream with the intermediate nicotinamide (up to 80% conversion). It was possible to select operating conditions that allowed long periods of operation (at least 100 h) at a constant flow-rate without enzyme activity loss.

• 出版日期2010-8-5