An Evaluation of the Effect of 17 alpha-Methyltestosterone Hormone on some Biochemical, Molecular and Histological Changes in the Liver of Nile Tilapia; Oreochromis niloticus

作者：Hasheesh Wafaa S; Marie Mohamed Assem S; Abbas Hossam H^*; Eshak Mariam G; Zahran Eman A

来源：Life Science Journal-Acta Zhengzhou University Overseas Edition, 2011, 8(3): 343-U1505.

• Nile tilapia
• 17 alpha- methyltestosterone
• sex reversal
• plasma constituents
• DNA alteration

The present field investigation was designed to explain clearly why methyltestosterone is widely used by the producers of farmed tilapia. Also to demonstrate why there are no known risks to consumers, producers and on the environment from using this hormone provided the recommended best practices for methyltestosterone used in aquaculture of fish. In this study, all water quality parameters were within the acceptable range for fish growth. The present analyses showed no significant differences in plasma total protein, albumin, globulin, A/G ratio, AST, ALT, LDH, it showed highly significant differences in plasma CPK activities. Molecular biological analyses revealed that using of methyltestosterone was able to induce DNA fragmentation and molecular genetic variability (using RAPD-PCR fingerprinting pattern) in the liver tissues of the treated Nile tilapia; Oreochromis niloticus, which was higher in the first four studied months than the untreated control tilapia. Additionally, histopathological examination in liver sections of control fish showed normal structure followed by diffuse severe hepatocytic vacuolations, the treated fish showed diffuse vacuolar degeneration followed by mild and severe hepatocytic vacuolations. [Wafaa S. Hasheesh, Mohamed- Assem S. Marie, Hassam H. Abbas, Mariam G. Eshak and Eman A. Zahran. An Evaluation of the Effect of 17 alpha-Methyltestosterone Hormone on some Biochemical, Molecular and Histological Changes in the Liver of Nile Tilapia; Oreochromis niloticus]. [Life Science Journal,. 2011; 8(3):343-358] (ISSN: 1097-8135). http://www.lifesciencesite.com.

• 出版日期2011