A new method for studying caffeine';s antioxygenic property: Peroxidase-Oxidase biochemical reaction

作者：Wang J; Cai RX^*; Lin ZX; Liu ZH

来源：Chinese Science Bulletin, 2003, 48(16): 1728-1732.

DOI：10.1360/02wb0204

• PO reaction
• HRP
• caffeine
• oscillation

The effect of Caffeine on Peroxidase-Oxidase (PO) reaction was studied systematically in this paper. We proved that the valley of PO oscillation is the best phase angle which was used to research antioxygenic property by the Analyte Pulse Perturbation Technique (APP), based on investigating the mechanism. Area integral calculus was proposed to use in quantitative analysis for the first time. There is a good linear relationship (R = 0.9950) between the ratio of amplitude changes of PO oscillation and the concentration of caffeine in the range 4.61 x 10(-7) mol/L-1.84 x 10(-5) mol/L. A new method for analysis by PO oscillation was set up. We also investigated two-dimensional projections and Fourier spectrum of nonlinear complicate system-PO reaction which was perturbed by caffeine, in order to provide a theoretical basis for studying effects of kinds of antioxidants on life system.

• 出版日期2003-8
• 单位武汉大学