Antinociceptive activity of Rhoifoline A from the ethanol extract of Zanthoxylum nitidum in mice

作者：Hu Jiang^*; Shi Xiaodong; Mao Xia; Chen Jiangang; Zhu Lei; Zhao Qingjie

来源：Journal of Ethnopharmacology, 2013, 150(3): 828-834.

DOI：10.1016/j.jep.2013.04.035

• Zanthoxylum nitidum
• Benzophenanthridine alkaloids
• Rhoifoline A
• Antinociception
• NO-cGMP
• L-type Ca2+ channels

Aim of the study: Antinociceptive activity of Rhoifoline A (RA), a benzophenanthridine alkaloid obtained from the ethanol extract of Zanthoxylum nitidum, was evaluated in mice using chemical and thermal models of nociception.
Materials and methods: RA was evaluated on anti-nociceptive activity in mice using chemical and thermal models of nociception.
Results: RA administered intraperitoneally at doses of 10, 20, 40 and 80 mg/kg exhibited significant inhibitions on chemical nociception induced by intraperitoneal acetic acid and subplantar formalin, and on thermal nociception in the tail-flick test and the hot plate test. RA neither significantly impaired motor coordination in the rotarod test nor did spontaneous locomotion in the open-field test. RA did not enhance the pentobarbital sodium induced sleep time. These results indicated that the observed antinociceptive activity of RA was unrelated to sedation or motor abnormality. Core body temperature measurement showed that RA did not affect temperature during a 2-hour period. Furthermore, RA-induced antinociception in the hot plate test was insensitive to naloxone or glibenclamide but significantly antagonized by L-NAME, methylene blue and nimodipine.
Conclusions: Therefore, it is reasonable that the analgesic mechanism of RA possibly involved the NO-cGMP signaling pathway and L-type Ca2+ channels.

• 出版日期2013-12-12
• 单位曲靖师范学院; 中国人民解放军第二军医大学