A fast and accurate isotope dilution GC-IT-MS/MS method for determination of eugenol in different tissues of fish: Application to a depletion study in mandarin fish

作者：Liu, Yongtao^*; Ai, Xiaohui; Li, Le; Li, Jincheng; Yang, Hong

来源：Biomedical Chromatography, 2018, 32(5): e4163.

DOI：10.1002/bmc.4163

• distribution and depletion
• eugenol
• GC-IT-MS
• MS
• isotope dilution
• mandarin fish

An accurate, rapid and effective method was established for determination of eugenol in plasma, muscle, skin, liver, kidney and gill of fish using gas chromatography-ion trap tandem mass spectrometry. Samples of muscle, skin, liver, kidney and gill were prepared using the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure, and a plasma sample was prepared by a liquid-liquid extraction procedure. Eugenol was monitored in <7min using an electron-ionization source in MS/MS mode and quantified by an internal standard of eugenol-d(3). The limit of detection was 5.0g/kg, and the limit of quantification was 10.0g/kg. The calibration curve was linear in the range of 5-1000g/L (R-2 = 0.9996). Intra- and inter-day precisions of eugenol expressed as relative standard deviation were within 9.74%, and the accuracy exhibited a relative error ranging from -2.20 to 8.89%. The developed method was successfully used to study the elimination regularity of eugenol in mandarin fish.

• 出版日期2018-5
• 单位中国水产科学研究院; 中国水产科学研究院长江水产研究所