Application of NMR-based metabonomics suggests a relationship between betaine absorption and elevated creatine plasma concentrations in catheterised sows

作者：Yde Christian Clement^*; Westerhuis Johan A; Bertram Hanne Christine; Knudsen Knud Erik Bach

来源：British Journal of Nutrition, 2012, 107(11): 1603-1615.

DOI：10.1017/S0007114511004909

• Absorption
• Betaine
• Dietary fibre
• Metabonomics
• NMR spectroscopy

The objective of the present explorative study was to determine the absorption dynamics when feeding diets varying in types and levels of dietary fibre in a catheterised animal model. A total of six sows were fed a diet low in fibre (LF), a diet high in soluble fibre and a diet high in insoluble fibre in a repeated 3 x 3 cross-over design. Plasma samples were collected from the mesenteric artery and the portal vein to determine different absorption phases by H-1 NMR spectroscopy-based metabonomics. Time profiles were determined for plasma levels of specific metabolites and for the absorption of these metabolites from the small intestine. The LF diet resulted in a higher betaine concentration in the blood than the two high-fibre diets (P=0.008). This leads to higher plasma concentrations of methionine (P=0.0028) and creatine (P=0.020) of endogenous origin. In conclusion, the use of NMR spectroscopy for measuring nutrient uptake in the present study elucidated the relationship between betaine uptake and elevated creatine plasma concentrations.

• 出版日期2012-6