Apoptosis and mammary health

作者：Lucker E^*; Mazurek S; Eigenbrodt E

来源：Tieraerztliche Praxis Ausgabe Grosstiere Nutztiere, 2000, 28(4): 182-188.

• apoptosis
• bovine mammary health
• involution
• mastitis
• milk yield depression
• poly(ADP-ribose) polymerase
• somatic cell count

Programmed cell death (apoptosis) is well established to be of central importance within numerous physiological and pathological processes. The role of apoptosis in bovine mammary health, however, has not been investigated. The present study gives an introductory review of apoptosis with special regard to mammary involution and health. In addition, we present preliminary results of immunological studies using poly(ADP-ribose) polymerase (PARP) as marker for apoptosis in mouse mammary tissue and bovine milk. Original PARP (116 kDa) and fragments thereof (54, 36, 27 kDa) were present in extracts of lactating mouse mammary glands. Following litter removal, PARP intensity was steadily decreased and lost at day six. In contrast, fragments at 27 kDa and 54 kDa increased starting with day two and day six, respectively. PARP was shown to be present in bovine milk. Major intensities were detected at 116, 95 and 41 kDa in milk from healthy udders. Milk as derived from udders affected by subclinical and clinical mastitis, showed an increased intensity at 41 kDa and additional fragments at 89, 64, and 36 kDa. These results indicate physiological and pathological bovine lactation to be associated with apoptotic cell loss. Further research in apoptosis must be taken to be important with regard to the characterization of bovine mammary health.

• 出版日期2000-7