Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

作者：Qiu Feng; Cao Jingyuan; Su Qiudong; Yi Yao; Bi Shengli^*

来源：International Journal of Molecular Sciences, 2014, 15(6): 9780-9788.

DOI：10.3390/ijms15069780

• multiplex TaqMan real-time PCR
• hepatitis A virus
• hepatitis E virus
• identification
• application

Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.

• 出版日期2014-6
• 单位中国疾病预防控制中心病毒病预防控制所