Acupuncture Decreases NF-kappa B p65, miR-155, and miR-21 and Increases miR-146a Expression in Chronic Atrophic Gastritis Rats

作者：Zhang, Jialing; Huang, Kangbai; Zhong, Guoxin; Huang, Yong^*; Li, Suhe^*; Qu, Shanshan; Zhang, Jiping

来源：Evidence-Based Complementary and Alternative Medicine, 2016, 2016: 9404629.

DOI：10.1155/2016/9404629

• HELICOBACTER-PYLORI INFECTION
• CHINESE MEDICINE
• MICRORNAS
• CLASSIFICATION
• MOXIBUSTION
• INDUCTION
• APOPTOSIS
• PROTEIN
• GROWTH

Acupuncture has been used to treat chronic atrophic gastritis (CAG) in traditional Chinese medicine (TCM) for centuries. In this study, we evaluated the effect of acupuncture at Zusanli (ST36), Zhongwan (0712), and Pishu (BL20) acupoints on weight changes of rats, histological changes of gastric glands, and expressions changes of nuclear factor -kappa B (NE -KB) p65, micro RNA- (miR-) 155, miR-21, and miR-146a in CAG rats induced by N-methyl-W-nitro-N-nitrosoguanidine (MNNG) combined with irregular diet. Consequently, we found that acupuncture treatment elevated body weight of rats significantly when compared to the model group. By observing histological changes, we found that the acupuncture group showed better improvement of gastric mucosa injury than the model group. Our results also demonstrated upregulation of NE -KB p65, miR-155, and miR-21 in gastric tissue of CAG rats and a positive correlation between miR-155 and miR-21. Relatively, expression of miR-146a was downregulated and negative correlation relationships between miR-146a and miR-155/miR-21 in CAG rats were observed. Additionally, expressions of NE -KB p65, miR-155, and miR-21 were downregulated and miR-146a was upregulated after acupuncture treatment. Taken together, our data imply that acupuncture can downregulate NE -KB p65, miR-155, and miR-21 and upregulate miR-146a expression in CAG rats. NE -KB p65, miR-155, miR-21, and miR-146a may play important roles in therapeutic effect of acupuncture in treating CAG.

• 出版日期2016
• 单位南方医科大学; 广州中医药大学