Assessment of the cytotoxicity of a mineral trioxide aggregate-based sealer with respect to macrophage activity

作者：Braga Julia Mourao; Oliveira Ricardo Reis; Martins Renata de Castro; Vieira Leda Quercia; Ribeiro Sobrinho Antonio Paulino

来源：Dental Traumatology, 2015, 31(5): 390-395.

DOI：10.1111/edt.12190

• macrophage
• mineral trioxide aggregate
• MTA Fillapex
• Viability
• Adherence
• phagocytosis

Aim: To assess the influence of co-culture with mineral trioxide aggregate (MTA) and MTA Fillapex (FLPX) on the viability, adherence, and phagocytosis activity of peritoneal macrophages from two mouse strains. Methodology: Cellular viability, adherence, and phagocytosis of Saccharomyces boulardii were assayed in the presence of capillaries containing MTA and MTA Fillapex. The data were analyzed using parametric (Student's t) and non-parametric (Mann-Whitney) tests. Results: FLPX was severely cytotoxic and decreased cell viability, adherence, and phagocytic activity of both macrophage subtypes. Cells that were treated with MTA Fillapex remained viable (>80%) for only 4 h after stimulation. Macrophages from C57BL/6 mice presented higher adherence and higher phagocytic activity compared with macrophages from BALB/c mice. Conclusion: Comparison of MTA and FLPX effects upon macrophages indicates that FLPX may impair macrophage activity and viability, while MTA seems to increase phagocytic activity.

• 出版日期2015-10