Dietary Probiotic Bacillus licheniformis TC22 Increases Growth, Immunity, and Disease Resistance, against Vibrio splendidus Infection in Juvenile Sea Cucumbers Apostichopus japonicus

作者：Zhao, Yancui^*; Yuan, Lei; Li, Mingzhu; Wan, Junli; Wang, Yiyan; Sun, Hushan

来源：Israeli Journal of Aquaculture – Bamidgeh, 2017, 69: 1415.

• Apostichopus japonicus
• probiotic
• growth
• immunity
• Bacillus

In this study we examined the effects of probiotic Bacillus licheniformis TC22 on growth, immunity, and disease resistance against Vibrio splendidus in juvenile sea cucumbers Apostichopus japonicus. For 30 days, sea cucumbers were fed diets with TC22 at 0 (control), 10(5), 10(7), and 10(9) CFU/g respectively. Results showed that dietary TC22 at 10(9) CFU/g significantly improved (P<0.05) growth of sea cucumbers. Dietary TC22 had no significant effects on total coelomocytes count, acid phosphatase activity, and superoxide dismutase activity in these animals (P>0.05). Dietary TC22 at 10(9) CFU/g significantly improved phagocytosis, and total nitric oxide synthase activity in sea cucumbers (P<0.05). Compared to the control, dietary TC22 had no significant effects on respiratory burst activity (P>0.05). Respiratory burst in sea cucumbers fed dietary TC22 at 10(9) CFU/g was significantly higher than those fed dietary TC22 at 10(7) CFU/g (P<0.05). Cumulative mortality after V. splendidus challenge decreased significantly in the sea cucumbers fed with TC22 at 10(9) CFU/g (P<0.05). The present study confirmed dietary B. licheniformis TC22 at 10(9) CFU/g could significantly improve immunity and disease resistance in juvenile A. japonicus.

• 出版日期2017
• 单位山东工商学院; 鲁东大学