A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells

作者：Lin, Bing; Yang, Xu-Zhong; Cao, Xue-Wei; Zhang, Tao-Zhu; Wang, Fu-Jun; Zhao, Jian^*

来源：Biotechnology Letters, 2017, 39(1): 71-78.

DOI：10.1007/s10529-016-2222-0

• Antitumor activity
• Apoptosis
• Drug delivery
• Heparin-binding EGF-like growth factor
• Trichosanthin

To evaluate the anti-tumor effects of trichosanthin after fusion with a cell penetrating peptide, heparin-binding peptide (HBP), derived from human heparin-binding EGF-like growth factor (HB-EGF).
The fusion protein of trichosanthin-HBP was expressed in Escherichia coli BL21 and purified by Ni-NTA affinity chromatography. The HBP domain had no influence on the topological inactivation activity and N-glycosidase activity of trichosanthin. Trichosanthin-HBP significantly inhibited the growth of tested cancer cells which are impervious to trichosanthin. Tumor cell apoptosis and both the mitochondrial- and death receptor-mediated apoptotic signaling pathways induced by trichosanthin-HBP were more significant than those induced by trichosanthin in HeLa cells.
HBP is an efficient intracellular delivery vehicle for trichosanthin and makes trichosanthin-HBP become a promising agent for cancer therapy.

• 出版日期2017-1
• 单位华东理工大学; 上海中医药大学; 生物反应器工程国家重点实验室