Nano polythionine-based electrochemiluminescence biosensor for detection of the p16INK4a gene using RuAg@AuNPs core-shell nanocomposites as DNA labels

作者：Wang, Yijie; Jiang, Meng; Shan, Yanqun; Jin, Xin; Gong, Miao; Wang, Xiaoying^*

来源：Journal of Luminescence, 2018, 201: 135-142.

DOI：10.1016/j.jlumin.2018.04.039

• The nano polythionine
• P16(INK4a) gene
• Electrochemiluminescence biosensor
• Core-shell nanocomposites

In this work, a nano polythionine (NPTh)-based universal sandwich-type electrochemiluminescence (ECL) biosensor for sensitive and specific detection of the p16(INK4a) gene using core-shell luminescent nanocomposites as excellent DNA labels was constructed. The NPTh electrode was prepared by in situ electropolymerization technique, which had a large surface area and was served as effective biosensing substrates for ssDNA1 immobilization by charge desorption. Meanwhile, tri(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+))/silver nanoparticles (AgNPs) doped gold (Au) core-shell nanocomposites (RuAg@AuNPs) were utilized as excellent ssDNA2 labels. The construction of dsDNA was fabricated through a hybridization reaction of ssDNA1, p16(INK4a) gene and RuAg@Au-ssDNA2. The change of ECL intensity on the NPTh electrode was found to have a linear relationship in respect to the logarithm of the p16(INK4a) gene concentrations in the wide range of 1.0x10(-13)similar to 1.0x10(-1) M, with a detection limit of 0.05 p.M. (S/N = 3). The presented biosensing strategy may provide great potential in the assay of other clinically biomarkers.

• 出版日期2018-9
• 单位东南大学