科研之友
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摘要
A simple but cost-effective method for preparing a novel partially carboxymethylated cotton gauze (PCG) with silver nanoparticles (AgNPs; i.e., PCG/Ag) was developed through carboxymethylation modification and reduction of Ag nitrate using polyvinylpyrrolidone (PVP) as a coating and complexing agent. The Fourier transform infrared spectrum of PCG/Ag revealed an absorption band at 1610 cm(-1) and no chemical conversion in the synthesis of AgNPs. The synthesis of spherical AgNPs with a diameter of 5-20 nm was confirmed by ultraviolet-visible spectroscopy and showed plasmon resonance peaks at 425 nm; X-ray diffraction analysis revealed peaks at 2 theta = 38.3 degrees and 44.2 degrees and X-ray photoelectron spectroscopy showed two peaks at 374.2-376.9 and 368.2-371.0 eV. AgNPs attached to partly carboxymethylated cotton gauze were optimized by a one-factor-at-a-time approach; the optimized conditions for an antimicrobial effect with no cytotoxicity was 30 min of soaking in 0.4% (w/v) Ag nitrate and reduction in 0.5% (w/v) ascorbic acid in the presence of defined amounts of PVP with stirring. PCG/Ag-4 showed excellent water absorption exceeding 2000% of the initial value. The inhibition zone test and bacterial growth inhibition curves suggested that PCG/Ag had antimicrobial activity against Escherichia coli and Staphylococcus aureus. The cell viability and cell attachment assays confirmed that the PCG/Ag was not cytotoxic and promoted wound healing in an animal model. These results indicate that PCG/Ag is a safe and effective material for wound dressing. This novel in-situ reduction method for obtaining uniformly dispersed nanoparticles on a biomaterial for wound dressing can be applied on a large scale without sacrificing low cost and efficiency.
- 出版日期2018-9
- 单位西南大学
