Determination of Tocopherols and Tocotrienols in Vegetable Oils by Nanoliquid Chromatography with Ultraviolet-Visible Detection Using a Silica Monolithic Column

作者：Cerretani Lorenzo; Jesus Lerma Garcia Maria; Manuel Herrero Martinez Jose; Gallina Toschi Tullia; Francisco Simo Alfonso Ernesto

来源：Journal of Agricultural and Food Chemistry, 2010, 58(2): 757-761.

DOI：10.1021/jf9031537

• Botanical origin
• nano-LC
• silica monolithic columns
• tocopherols
• tocotrienols
• vegetable oils

A method for the determination of tocopherols and tocotrienols in vegetable oils by nanoliquid chromatography with UV-vis detection has been developed. The separation of tocopherols was optimized in terms of mobile phase composition on the basis of the best compromise between efficiency, resolution, and analysis time. The optimal conditions were achieved using a C18 silica monolithic column (150 mm x 0.1 mm) with an isocratic elution of acetonitrile/methanol/water (acidified with 0.2% acetic acid) at a flow rate of 0.5 mu L min(-1), giving a total analysis time below 18 min. The method has been applied to the quantification of tocopherols and tocotrienols present in several vegetable oils with different botanical origins.

• 出版日期2010-1-27