DEVELOPMENT OF INTRON TARGETING (IT) MARKERS FOR POTATO AND CROSS-SPECIES AMPLIFICATION IN SOLANUM NIGRUM (SOLANACEAE)

作者：Poczai Peter^*; Cernak Istvan; Gorji Ahmad Mosapour; Nagy Sandor; Taller Janos; Polgar Zsolt

来源：American Journal of Botany, 2010, 97(12): E142-E145.

DOI：10.3732/ajb.1000360

• genetic diversity
• intron targeting
• potato
• Solanum nigrum
• transferability

Premise of the study : Intron Targeting (IT) primers were developed for potato using expressed sequence tags (EST) and NCBI database records to study genetic diversity.
Methods and Results : Twenty-nine polymorphic intron targeting (IT) markers were generated and characterized from 30 samples of potato and 22 samples of Solanum nigrum to detect polymorphism. The number of alleles (A) per locus ranged from 2 to 7 in the analyzed populations, and the observed heterozygosity (H-O) and expected heterozygosity (H-E) from 0 to 0.833 and 0.750, respectively. All of the primers also amplified in the related species S. nigrum.
Conclusions : The developed markers will provide valuable tools for genetic diversity analysis, genetic mapping, and marker-assisted breeding of potato and related Solanum species.

• 出版日期2010-12