A Microarray-Based Gene Expression Analysis to Identify Diagnostic Biomarkers for Unknown Primary Cancer

作者：Kurahashi Issei; Fujita Yoshihiko; Arao Tokuzo; Kurata Takayasu; Koh Yasuhiro; Sakai Kazuko; Matsumoto Koji; Tanioka Maki; Takeda Koji; Takiguchi Yuichi; Yamamoto Nobuyuki; Tsuya Asuka; Matsubara Nobuaki; Mukai Hirofumi; Minami Hironobu; Chayahara Naoko; Yamanaka Yasuhiro; Miwa Keisuke; Takahashi Shin; Takahashi Shunji; Nakagawa Kazuhiko; Nishio Kazuto^*

来源：PLos One, 2013, 8(5): e63249.

DOI：10.1371/journal.pone.0063249

Background: The biological basis for cancer of unknown primary (CUP) at the molecular level remains largely unknown, with no evidence of whether a common biological entity exists. Here, we assessed the possibility of identifying a common diagnostic biomarker for CUP using a microarray gene expression analysis. %26lt;br%26gt;Methods: Tumor mRNA samples from 60 patients with CUP were analyzed using the Affymetrix U133A Plus 2.0 GeneChip and were normalized by asinh (hyperbolic arc sine) transformation to construct a mean gene-expression profile specific to CUP. A gene-expression profile specific to non-CUP group was constructed using publicly available raw microarray datasets. The t-tests were performed to compare the CUP with non-CUP groups and the top 59 CUP specific genes with the highest fold change were selected (p-value%26lt;0.001). %26lt;br%26gt;Results: Among the 44 genes that were up-regulated in the CUP group, 6 genes for ribosomal proteins were identified. Two of these genes (RPS7 and RPL11) are known to be involved in the Mdm2-p53 pathway. We also identified several genes related to metastasis and apoptosis, suggesting a biological attribute of CUP. %26lt;br%26gt;Conclusions: The protein products of the up-regulated and down-regulated genes identified in this study may be clinically useful as unique biomarkers for CUP.

• 出版日期2013-5-9
• 单位中国医学科学院肿瘤医院