The consumption of n-3 polyunsaturated fatty acids differentially modulates gene expression of peroxisome proliferator-activated receptor alpha and gamma and hypoxia-inducible factor 1 alpha in subcutaneous adipose tissue of obese adolescents

作者：Mejia Barradas Cesar M; Del Rio Navarro Blanca E; Dominguez Lopez Aaron; Campos Rodriguez Rafael; Martinez Godinez Maria de Los A; Rojas Hernandez Saul; Lara Padilla Eleazar; Abarca Rojano Edgar; Miliar Garcia Angel^*

来源：Endocrine, 2014, 45(1): 98-105.

DOI：10.1007/s12020-013-9941-y

• Oxidative stress
• Hypoxia
• Adolescents
• Obesity
• n-3 PUFA

The aim of this study was to evaluate the effect of long-chain omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation on metabolic state and gene expression in subcutaneous adipose tissues of obese adolescents. Obese adolescents (n = 26, 10 girls and 16 boys) aged 12.4 +/- 2.1 years were assigned to a 12-week regimen of n-3 PUFA intake. Five times per day, subjects received a food supplement consisting of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (3 g per day, 944 mg EPA, and 2,088 mg DHA). Blood parameters were measured, and subcutaneous adipose tissue biopsies were analyzed to determine gene expression at baseline and after 12 weeks. Student's t test and the Wilcoxon signed-rank test were used to estimate differences in arithmetic means of pre- and post-dietary supplementation for various anthropometric, biochemical, clinical, and gene expression parameters. After 12 weeks, n-3 PUFA consumption was associated with decreased body mass index (29.7 +/- 4.6 vs. 27.8 +/- 4.4 kg/m(2); P < 0.001), waist circumference (93.2 +/- 9.9 vs. 90.5 +/- 10.0 cm; P < 0.003), hip circumference (102.9 +/- 10.9 vs. 101.1 +/- 10.9 cm; P < 0.014), and blood triglyceride levels (220.8 +/- 27.4 vs. 99.7 +/- 32.7 mg/dL; P < 0.001). Fatty acid supplementation/n3 PUFA supplementation was associated with a downregulated expression of the genes encoding PPAR gamma and PGC-1 alpha (P < 0.001), and an up-regulated expression of the genes encoding PPAR alpha (P < 0.007) and SREBP1 (P < 0.021). The expressions of SOD2 (P < 0.04), CAT (P < 0.001), GPX3 (P < 0.032) and HIF-1 alpha protein also decreased. Our study demonstrated that n-3 PUFA consumption and dietary restriction improved the anthropometric parameters and decreased the triglycerides levels of the adolescents, suggesting a reduction in hypoxia in subcutaneous adipose tissue.

• 出版日期2014-2