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摘要
The adsorption behavior of bovine serum albumin (BSA) on poly(acryloyl pyrrole) (PAP) was investigated in detail. Firstly, PAP, which was polymerized by free radical polymerization, was spin-coated onto 50 nm thick Au films to form the homogeneous surfaces. Afterwards, the chemical structures and group contents were characterized by Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). And the surface wettability of PAP in buffer solutions with different pH values was detected by the contact angle measurement. Secondly, the adsorption behavior of BSA on PAP was studied in situ by surface plasmon resonance spectroscopy (SPR). Herein, the factors to affect the adsorption of protein mainly include the pH values of buffer solutions and concentration of BSA used. The interactions between the protein molecules and polymer films changed in various buffer solution circumstances. Consequently, the adsorption behavior and the adsorbed amounts would be varied. PAP could be supplied as a promising material for fabricating more sensitive protein biochips in the recent future.
- 出版日期2009-9-14
- 单位郑州轻工业大学
