Altered MicroRNA Expression Profiles in Activated Mast Cells Following IgE-Fc epsilon RI Cross-Linking with Antigen

作者：Teng Yaoshu; Zhang Ruxin^*; Yu Hongzhi; Wang Hong; Hong Zhicong; Zhuang Wenjie; Huang Yu

来源：Cellular Physiology and Biochemistry, 2015, 35(6): 2098-2110.

DOI：10.1159/000374016

• MiRNA
• Microarray
• Mast cell
• Immunoglobulin E
• Fc receptor

Background/Aims: MicroRNAs (miRNAs) are critical regulators of immune responses and immunologic disorders. However, little is known about miRNA expression and function during mast cell differentiation, proliferation and activation. This study aimed to determine the miRNA expression profiles in mast cells stimulated by immunoglobulin E (IgE) and antigen and to analyze the potential functions of specific miRNAs. Methods: Bone marrow-derived mast cells (BMMCs) generated from differentiated mouse bone marrow cells were untreated (Unstimu) or stimulated with IgE-antigen complexes for 1 h or 6 h (Stimu). The miRNA profiles were evaluated by miRNA microarray. MiRNA target gene prediction and enrichment analyses were performed using bioinformatics. Results: Seven significantly up-regulated and 10 down-regulated miRNAs were identified in the 1 h Stimu group relative to the Unstimu group (fold change>2; P<0.05). Of 8 miRNAs randomly selected from the 17 identified, the expression levels of 6 were confirmed by quantitative real-time PCR (qRT-PCR). The potential target genes of several candidate miRNAs were enriched in Fc epsilon RI signaling, response to stimulus and cellular exocytosis. Conclusion: The expression of many miRNAs changes following IgE-Fc epsilon RI cross-linking in activated mast cells, and these miRNAs probably play key regulatory roles in core signaling pathways and biological behaviors. Evaluating the functions of these characteristic miRNAs will further our understanding of IgE-associated allergic disease pathogenesis and the development of therapeutic strategies.

• 出版日期2015
• 单位复旦大学