Application of retrovirus-mediated expression cloning for receptor screening of a parasite

作者:Kobayashi Kyousuke; Kato Kentaro*; Sugi Tatsuki; Yamane Daisuke; Shimojima Masayuki; Tohya Yukinobu; Akashi Hiroomi
来源:Analytical Biochemistry, 2009, 389(1): 80-82.
DOI:10.1016/j.ab.2009.03.008

摘要

Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.