Delivery of Helicobacter pylori HpaA to gastrointestinal mucosal immune sites using Lactococcus lactis and its immune efficacy in mice

作者：Zhang, Rongguang; Wang, Chen; Cheng, Wenbin; Duan, Guangcai^*; Shi, Qingfeng; Chen, Shuaiyin; Fan, Qingtang

来源：Biotechnology Letters, 2018, 40(3): 585-590.

DOI：10.1007/s10529-017-2502-3

• Adhesin
• Genetic engineering
• Helicobacter pylori
• Lactococcus lactis
• Mucosal immune

To develop a safe and effective oral vaccine against Helicobacter pylori using its HpaA protein expressed in Lactococcus lactis. @@@ The gene encoding HpaA was obtained by PCR and ligated to pNZ8110-lysM following digestion with NaeI + SphI. The recombinant plasmid was transferred into E. coli for multiplication, and then into L. lactis. The recombinant L. lactis was induced to express HpaA, resulting in two products of 29 and 25 kDa, both of which yielded positive immunoreaction with mouse antisera against H. pylori, as confirmed by immunoblot assays. The 29 kDa product constituted 12% of the cell lysates. Oral inoculation with the engineered L. lactis evoked significantly elevated serum IgG level in mice (P < 0.05). @@@ A novel engineered L. lactis strain was developed that efficiently produces whole HpaA protein with desired antigenicity and potent immunogenicity. It provides a basis for approaches to L. lactis-delivered anti-H. pylori vaccination.

• 出版日期2018-3
• 单位新乡医学院; 郑州大学