ATM regulates a DNA damage response posttranscriptional RNA operon in lymphocytes

作者：Mazan Mamczarz Krystyna; Hagner Patrick R; Zhang Yongqing; Dai Bojie; Lehrmann Elin; Becker Kevin G; Keene Jack D; Gorospe Myriam; Liu Zhenqui; Gartenhaus Ronald B^*

来源：Blood, 2011, 117(8): 2441-2450.

DOI：10.1182/blood-2010-09-310987

Maintenance of genomic stability depends on the DNA damage response, a biologic barrier in early stages of cancer development. Failure of this response results in genomic instability and high predisposition toward lymphoma, as seen in patients with ataxia-telangiectasia mutated (ATM) dysfunction. ATM activates multiple cell-cycle checkpoints and DNA repair after DNA damage, but its influence on posttranscriptional gene expression has not been examined on a global level. We show that ionizing radiation modulates the dynamic association of the RNA-binding protein HuR with target mRNAs in an ATM-dependent manner, potentially coordinating the genotoxic response as an RNA operon. Pharmacologic ATM inhibition and use of ATM-null cells revealed a critical role for ATM in this process. Numerous mRNAs encoding cancer-related proteins were differentially associated with HuR depending on the functional state of ATM, in turn affecting expression of encoded proteins. The findings presented here reveal a previously unidentified role of ATM in controlling gene expression posttranscriptionally. Dysregulation of this DNA damage response RNA operon is probably relevant to lymphoma development in ataxiatelangiectasia persons. These novel RNA regulatory modules and genetic networks provide critical insight into the function of ATM in oncogenesis. (Blood. 2011;117(8):2441-2450)

• 出版日期2011-2-24
• 单位NIH