Effects of Atractylodes macrocephala Koidzumi rhizome on 3T3-L1 adipogenesis and an animal model of obesity

作者：Kim Chang Keun; Kim Mihyun; Oh Sang Deog; Lee Sang Min; Sun Boram; Choi Gi Soon; Kim Sun Kwang; Bae Hyunsu; Kang Chulhun; Min Byung Il^*

来源：Journal of Ethnopharmacology, 2011, 137(1): 396-402.

DOI：10.1016/j.jep.2011.05.036

• Atractylodes macrocephala Koidzumi
• Obesity
• 3T3-L1 adipocyte
• Oil Red O staining
• Phospho-Akt
• Perilipin

Ethnopharmacological relevance: Atractylodes macrocephala Koidzumi (AMK) is an herbal medicine traditionally used for treatment of abdominal pain, gastrointestinal disease, obesity, and related complications.
Aim of the study: We investigated the effects and molecular mechanism of AMK rhizome water extract on 3T3-L1 adipogenesis and an animal model of obesity.
Materials and methods: To study the effect of AMK on adipogenesis in vitro, differentiating 31341 cells were treated every two days with AMK at various concentrations (1-25 mu g/ml) for eight days. Oil Red 0 staining was performed to determine the lipid accumulation in 3T3-L1 cells. To elucidate the inhibitory mechanism of AMK on adipogenesis, phosphorylation levels of Akt and expression of perilipin, were analyzed by Western blotting. AMK was administered orally to high fat diet (HFD)-induced obese rats to confirm its effect in vivo.
Results: AMK inhibited 3T3-L1 adipocyte differentiation in a dose-dependent manner without cellular toxicity. Phospho-Akt expression was highly decreased by AMK treatment, whereas there was no significant change in perilipin expression. AMK administration significantly reduced the body weight of rats fed a HFD. Plasma triglyceride levels were significantly lower in the AMK-treated HFD group than those in the HFD control group or normal diet (ND) group, although serum total, HDL- and LDL-cholesterol levels did not differ between the groups.
Conclusion:These results demonstrate an inhibitory effect of AMK on adipogenesis through reduction of an adipogenic factor, phospho-Akt. AMK had a beneficial effect, reducing body weight gain in a HFD-induced animal model of obesity.

• 出版日期2011-9-1