A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

作者：Iturbe Ormaetxe Inaki; Woolfit Megan; Rances Edwige; Duplouy Anne; O'Neill Scott L^*

来源：Journal of Microbiological Methods, 2011, 84(1): 134-136.

DOI：10.1016/j.mimet.2010.10.019

• Wolbachia pipientis
• Purification
• Genomics
• Symbiosis

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.

• 出版日期2011-1