A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus)

作者:Sun Boguang; Zhang Xiao Hua*; Tang Xuexi; Wang Shushan; Zhong Yingbin; Chen Jixiang; Austin Brian
来源:Journal of Bacteriology, 2007, 189(6): 2575-2579.
DOI:10.1128/JB.01650-06

摘要

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.