Application of loop-mediated isothermal amplification for detection of Yersinia enterocolitica in pork meat

作者：Gao Hongwei^*; Lei Zhiwen; Jia Juntao; Wang Shufeng; Chen Ying; Sun Min; Liang Chengzhu

来源：Journal of Microbiological Methods, 2009, 77(2): 198-201.

DOI：10.1016/j.mimet.2009.02.001

• Loop-mediated isothermal amplification
• Detection
• Yersinia enterocolitica

A loop-mediated isothermal amplification (LAMP) method was developed for the detection of Yersinia enterocolitica isolates in both pure bacterial cultures and pork meat. The LAMP primers, which corresponded to the gyrB gene, accurately identified 4 different bioserotypes of Y. enterocolitica. These primers failed to detect Y. pseudotuberculosis, Y. frederiksenii, and 17 non-Yersinia strains. The sensitivity of the LAMP assay for the detection of Y. enterocolitica in pure culture was 65 CFU/mL (31.6 fg of genomic DNA). The LAMP assay was conducted for the detection of Y. enterocolitica strains in 21 pig tonsil samples and 73 pork meat samples obtained from 94 slaughtered pigs belonging to 4 different herds. Y. enterocolitica was found to be present in 4 tonsil samples and none in meat samples. This is the first report in which the LAMP assay was employed for the detection of Y. enterocolitica in food samples.

• 出版日期2009-5
• 单位中华人民共和国山东出入境检验检疫局; 中国检验检疫科学研究院