Application of UPLC-Quadrupole-TOF-MS Coupled with Recycling Preparative HPLC in Isolation and Preparation of Coumarin Isomers with Similar Polarity from Peucedanum praeruptorum

作者：Lv, Huawei; Luo, Jianguang; Wang, Xiaobing; Kong, Lingyi^*

来源：Chromatographia, 2013, 76(3-4): 141-148.

DOI：10.1007/s10337-012-2368-4

• UPLC-Q-TOF-MS
• Recycling preparative HPLC
• Coumarin isomers
• Peucedanum praeruptorum

A strategy of ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) coupled to recycling preparative HPLC was utilized to sensitively detect the minor constituents and preparatively isolate coumarin isomers from the petroleum ether extract of Peucedanum praeruptorum roots. UPLC-Q-TOF-MS was achieved on an Agilent ZORBAX Extend C-18 analytical column (50 mm x 2.1 mm i.d. 1.8 mu m) using methanol:H2O as the mobile phase, and three pairs of coumarin isomers with similar polarity were detected. Then recycling preparative HPLC was successfully used in one step for the isolation and purification of these six compounds with optimized mobile phases (methanol: H2O, 73:27, 81:19, 78:22, v/v). The structures of six coumarins including three new coumarins named praeruptorin (F-H) (1, 3 and 6) were identified by spectroscopic analysis.

• 出版日期2013-2
• 单位天然药物活性组分与药效国家重点实验室; 中国药科大学