Apoptotic Effect of Sanggenol L via Caspase Activation and Inhibition of NF-B Signaling in Ovarian Cancer Cells

作者：Nam Moon Sik; Jung Deok Beom; Seo Kyeong Hwa; Kim Bo Im; Kim Ju Ha; Kim Jung Hyo; Kim Bonglee; Baek Nam In; Kim Sung Hoon^*

来源：Phytotherapy Research, 2016, 30(1): 90-96.

DOI：10.1002/ptr.5505

• sanggenol L
• ovarian cancer cells
• apoptosis
• PARP
• caspase 9
• 3
• NF-B

In the present study, the underlying apoptotic mechanism of sanggenol L was elucidated in ovarian cancer cells. Sanggenol L showed cytotoxic and antiproliferative effect in A2780, SKOV-3, and OVCAR-3 ovarian cancer cells in a concentration-dependent fashion. Consistently, sanggenol L increased sub-G1 phase population and early and late apoptotic portion in ovarian cancer cells. Also, sanggenol L activated caspase9/3, suppressed the phosphorylation of IB and p65 NF-B (nuclear factor kappa-light-chain-enhancer of activated B cells), attenuated the expression of Cyclin D1, and cleaved poly(adenosine diphosphate ribose -ribose) polymerase in SKOV-3, A2780, and OVCAR-3 cells. Furthermore, sanggenol L blocked nuclear translocation of NF-B and also attenuated the expression of NF-B related genes such as c-Myc, Cyclin D1, and Bcl-L-X, Bcl-2, in lipopolysaccharide-treated SKOV-3 cells. Overall, our findings for the first time suggest that sanggenol L induces apoptosis via caspase activation and inhibition of NF-B/IB phosphorylation as a potent chemotherapeutic agent for ovarian cancers.

• 出版日期2016-1