5,5 '-Substituted Indirubin-3 '-oxime Derivatives as Potent Cyclin-Dependent Kinase Inhibitors with Anticancer Activity

作者：Choi Soo Jeong; Lee Jung Eun; Jeong Soon Young; Im Isak; Lee So Deok; Lee Eun Jin; Lee Sang Kook; Kwon Seong Min; Ahn Sang Gun; Yoon Jung Hoon; Han Sun Young; Kim Jae Il; Kim Yong Chul^*

来源：Journal of Medicinal Chemistry, 2010, 53(9): 3696-3706.

DOI：10.1021/jm100080z

To enhance the ability of indirubin derivatives to inhibit CDK2/cyclin E, a target of anticancer agents, we designed and synthesized a new series of indirubin-3'-oxime derivatives with combined substitutions at the 5 and 5' positions. A molecular docking study predicted the binding of derivatives with OH or halogen substitutions at the 5' position to the ATP binding site of CDK2, revealing the critical interactions that may explain the improved CDK2 inhibitory activity of these derivatives. Among the synthesized derivatives, the 5-nitro-5'-hydroxy analogue 3a and the 5-nitro-5'-fluoro analogue Sa displayed potent inhibitory activity against CDK2, with IC(50) values of 1.9 and 1.7 nM, respectively. These derivatives also showed antiproliferative activity against several human cancer cell lines, with IC(50) values of 0.2-3.3 mu M. A representative analogue, 3a, showed greater than 500-fold selectivity for CDK relative to selected kinase panel and potent in vivo anticancer activity.

• 出版日期2010-5-13