Ultimate Physical Characterization of Biocomposites Constituted by Enzymes on Template of Organized Molecular Films of Organo-Modified Aluminosilicate with High Surface Coverage

作者：Arai Shuntaro; Fujimori Atsuhiro^*

来源：Sen-I Gakkaishi, 2014, 70(6): 109-113.

DOI：10.2115/fiber.70.109

Organized molecular films adsorbed several bio-materials have been prepared by Langmuir-Blodgett method using an organo-modified. montmorillonite. In this method, organo-montmorillonite films play a template role of the lysozyme thin layer. Chemisorption of lysozyme to the anionic montmorillonite surface has supported by infrared spectra of multilayers. The bands of N-H bending and C=O stretching vibrations of lysozyme were clearly identified in the spectra of multilayers coated with chemisorbed lysozyme. The morphology of these film surfaces were observed by atomic force microscopy at mesoscopic scales. In the case of organo-modified montmorillonite adsorbed protease, it is also indicated that activity of chemisorbed protease was maintained up to 160 degrees C of annealing temperature. The thickness of the enzyme layer was also maintained. This conservation of steric structure of enzyme is ascribable to the formation of aggregate of adsorbed enzyme on the montmorillonite surface and the construction of sandwiched structure of enzyme between organo-montmorillonite..

• 出版日期2014-6