Molecular basis for antifreeze activity difference of two insect antifreeze protein isoforms

作者：Zhou YanXia; Tan HongWei; Yang ZuoYin; Jia ZongChao; Liu RuoZhuang; Chen GuangJu^*

来源：Science in China Series B-Chemistry, 2007, 50(2): 266-271.

DOI：10.1007/s11426-007-0027-7

• insect antifreeze protein
• synergistic effect
• antifreeze activity
• beta-helix
• quantum chemistry
• molecular mechanics
• molecular dynamics

The insect spruce budworm (Choristoneura fumiterana) produces antifreeze protein (AFP) to assist in the protection of the over-wintering larval stage and contains multiple isoforms. Structures for two isoforms, known as CfAFP-501 and CfAFP-337, show that both possess similar left-handed beta-helical structure, although thermal hysteresis activity of the longer isoform CfAFP-501 is three times that of CfAFP-337. The markedly enhanced activity of CfAFP-501 is not proportional to, and cannot be simply accounted for, by the increased ice-binding site resulting from the two extra coils in CfAFP-501. In order to investigate the molecular basis for the activity difference and gain better understanding of AFPs in general, we have employed several different computational methods to systematically study the structural properties and ice interactions of the AFPs and their deletion models. In the context of intact AFPs, a majority of the coils in CfAFP-501 has better ice interaction and causes stronger ice lattice disruption than CfAFP-337, strongly suggesting a cooperative or synergistic effect among beta-helical coils. The synergistic effect would play a critical role and make significant contributions to the antifreeze activity beta-helical antifreeze proteins. This is the first time that synergistic effect and its implication for antifreeze activity are reported for beta-helical antifreeze proteins.

• 出版日期2007-4
• 单位北京师范大学; 北京化工大学